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KMID : 0366119770050030133
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Korean Journal of Applied Microbiology & Bioengineering
1977 Volume.5 No. 3 p.133 ~ p.140
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The Physiological and Biochemical Studies of Nocardia sp
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Abstract
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The results of electron microscopic studies on the cell fine structure of Nocardia sp the location of tellurite-reducing enzyme and the reduction part of T. T. C. (Triphenyl tetrazonium chloride) were summariz as follows.
As the fine structure of the cell, the membrane-like structure with unit membrane was distributed in the cytoplasm. The membrane-like structure had complicate forms; some of membrane-like structure appeared spiral form.
As the metal tellurium salt appeared in the cytoplasm, it is obvious that tellurite and tellurate-reducing enzymes are present in the cytoplasm.
Reduction of T. T. C. took place in the cell membrane and the intracellular membrane-like structure. Therefore, it was thought that reduction of tellurate and T. T. C. took place in different parts.
T. T C. formazane formed in the cell was reoxidized by osmic acid which was used as a fixation reagent for the electron microscopic specimen preparation. As 95% T. T. C. formazane was soluble in ethanol and embedding materials and removed out of the cell, an originally formed formazane appeared as electron light part on the electron microscopic image.
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